The Role of Dpnase in the Mechanism of Action of an Antitumor Alkylating Agent on Ehrlich Ascites Cells.

3 Jacob, T. MI., E. Ohtsuka, M. W. Moon, S. A. Narang, and H. G. Khorana, Federation Proc., 23, 531 (1964). 4Jacob, T. M., and H. G. Khorana, Abstr. Sixth Internat. Congr. Biochem., 1, 62 (1964). 5 Most of the abbreviations are as in current use in J. Biol. Chem.: d-(TTC)3 is abbreviation for the nonanucleotide d-TpTpCpTpTpCpTpTpC, bearing no phosphomonoester end group, and similarly, d-(TTC)2 and d-(TTC)4 are homologues containing, respectively, 2 and 4 triplets; d-(TTC)3-TT is undecanucleotide, bearing at the right-hand terminus the fragment -TpT; poly AAG, ribopolynucleotide containing the repeating sequence, adenylyl-adenylyl-guanylate. 6 Chamberlin, M., and P. Berg, these PROCEEDINGS, 48, 81 (1962). 7 Chamberlin, M., and P. Berg, J. Mol. Biol., 8, 708 (1964). 8 Mehrotra, B. D., and H. G. Khorana, J. Biol. Chem., in press. 9 Bollum, F. J, J.Biol. Chem., 234, 2733 (1959); Mans, R. J., and G. D. Novelli, Arch. Biochem. Biophys., 94, 48 (1961). 10 We are grateful to Drs. K. Takahashi, Tokyo University, P. Berg, Stanford University, and L. A. Heppel, National Institutes of Health, for gifts of preparations of this enzyme made in their laboratories. 11 Bremer, H., and M. W. Konrad, these PROCEEDINGS, 51, 801 (1964). 12 Stevens, A., J. Biol. Chem., 239, 204 (1964). 13 Fox, C. F., and S. B. Weiss, J. Biol. Chem., 239, 175 (1964). 14 Kornberg, A., L. R. Bertsch, J. Jackson, and H. G. Khorana, these PROCEEDINGS, 51, 315 (1964).